Advanced Flow and TUNEL Assays

The first specimen that was selected on the Assay Selection tab will appear in the Specimen Info box at the top of the Results tab screen. The Advanced Flow and TUNEL assays have identical data entry screens. You can use the database navigation buttons to switch between specimens that were selected on the Assay Selection tab.

Figure: Advanced Flow and TUNEL assay screens



  1. Select whether or not results for the specimen were obtained from the Were results obtained on this specimen radio button.

  2. Indicate whether or not the sample was fresh or frozen in the Indicate whether the assay was performed on a fresh or frozen specimen box.

  3. Select the gating method from the Indicate the Gating method used for anchor Immunophenotyping drop-down.

    • Method 1 gates all lymphocytes using FL/SS followed by a second gate on fluorescence histogram to select for the anchor marker.

    • Method 2 gates the anchor marker fluorescence versus SS histogram.

  4. Complete all of the rows and columns Anchor Gated Analysis section.

    • For Anchor Gate <500 cells, LDMS will auto-fill subsequent rows based on your selections for the first row of data. You can manually change individual marker sets in other rows if needed.

    • Rows can be added or deleted by using the Add Row and Delete Row buttons to the right.

    • If a marker was not run, select the N/A check box at the end of the row and select the appropriate Censor from the drop-down.

    • The Total Percent field will automatically be calculated. The sum of the four quandrants should equal 100%; if it does not, the Total Percent will turn red.

  5. Enter the subset marker and colors, and quadrant results into the Total Lymphocyte Gated Analysis fieldset.

  6. Enter the assay technician’s initials into the Assay Tech field.

  7. Enter your initials into the Data Entered By field.

  8. Click the button from the LDMS toolbar.

To view the Advanced Flow Patient Report after entering data for the specimens tested, click the button from the LDMS toolbar.

After you've finished with the first specimen, click the button from the LDMS tool bar and enter the results for the next specimen. Once all specimens have been entered and added, the data entry is complete. You can now return to the Assay Selection tab to perform more work.